Alterations in oxidative stress parameters and its associated correlation with clinical disease on experimental Cryptosporidium parvum infection in Swiss albino mice.

Abstract

The present study was conducted to evaluate the changes in oxidative stress parameters on experimental infection with Cryptosporidium parvum in Swiss albino mice. The mice were divided into four groups viz., group I-IV, each group comprising of 15 mice. Group I mice served as healthy control. In Group II mice, C. parvum oocysts @ 104/os were administered, mice of group III were given dexamethasone @ 30µg/ml in drinking water whereas group IV mice were given dexamethasone @ 30µg/ml along with C. parvum oocysts @ 104/os. Significant changes were seen in oxidative stress parameters which included significant increase in LPO and decrease in levels of SOD, CAT and GSH in liver and intestine in group IV mice at 10th DPI when compared to others indicating an important role played by free radical induced oxidative stress in the development of C. parvum infection in mice which was clinically characterized by loss of body condition, profuse bloody diarrhoea and peak oocyst shedding intensity occurring at 10th DPI.