Abstract
BackgroundHepatocellular carcinoma (HCC) can lead to liver failure which renders to liver transplant. miRNAs might be detected as biomarkers in subclinical stage of several hepatobiliary disorders like HCC. Therefore, in the present study, alterations in miRNAs as biomarkers were detected in LT patients with HCC.MethodsFourteen tissue samples composed of 5 rejected and 9 non-rejected ones were used for studying the miRNAs expression pattern using LNA-array probe assay and the result was evaluated by in house SYBR Green Real-time PCR protocols on 30 other tissue samples composed of 10 rejected and 20 non-rejected ones for the selected miRNAs. All samples were collected from liver transplanted patients with HCC.ResultsThe study results revealed that in rejected patients compared to non-rejected ones, hsa-miR-3158-5p, -4449, -4511, and -4633-5p were up-regulated and hsa-miR-122-3p, -194-5p, 548as-3p, and -4284 were down-regulated. ROC curve analysis also confirmed that miR194-5p and -548as-3p in up-regulated and also, miR-3158-5p, -4449 in down-regulated microRNAs are significantly important molecules in rejection.ConclusionFinally, the tissue levels of specific miRNAs (especially hsa-miR-3158-5p, -4449, -194-5p and -548as-3p) significantly correlated with the development of HCC, which can be present as biomarkers after further completing studies.
Highlights
Hepatocellular carcinoma (HCC) can lead to liver failure which renders to liver transplant. miRNAs might be detected as biomarkers in subclinical stage of several hepatobiliary disorders like HCC
A total number of 44 formalin-fixed paraffin-embedded (FFPE) samples were collected from liver transplant (LT) patients with HCC
Descriptive statistics The test group was composed of 14 LT patients with the mean age of 47.4 ± 7.8 years and included 9 (64.3%) males
Summary
Hepatocellular carcinoma (HCC) can lead to liver failure which renders to liver transplant. miRNAs might be detected as biomarkers in subclinical stage of several hepatobiliary disorders like HCC. Hepatocellular carcinoma (HCC) can lead to liver failure which renders to liver transplant. A considerable part of human genome is consisted of non-coding regions, including non-coding RNAs, known as microRNAs (miRNAs) [1]. These regions encode dynamic miRNAs, regulating around 20–30% of mammalian genes that comprise nearly 5% of transcriptome [1, 5]. MiRNAs are evolutionary conserved, made up of 18–25 nucleotides They are involved in various biological processes e.g. cell growth, apoptosis, hematopoietic lineage differentiation, and gene regulation [1, 6, 7]
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