Alterations in gut microbiota of abdominal aortic aneurysm mice

Jiahe Xie,Yuhua Hu,Zuheng Liu,Dongming Xie,Zhonggao Zhong,Ziyou Liu,Lintao Zhong,Bo Ye,Weiling Lu,Qingrui Li,Hai Xiao,Rongming Ding,Guofu Zheng,Yiming Zhong

doi:10.1186/s12872-020-01334-2

Abstract

BackgroundThe gut microbiome plays an important role in various cardiovascular diseases, such as atherosclerosis and hypertension, which are associated with abdominal aortic aneurysms (AAAs).MethodsHere, we used 16S rRNA sequencing to explore gut microbiota in C57BL ApoE−/− mice with AAAs. A mouse model of abdominal aortic aneurysms was induced with angiotensin II (Ang II) (1000 ng/min per kg). On day 28 after the operation, fecal samples were collected and stored at − 80 °C until DNA extraction. We determined the relative abundances of bacterial taxonomic groups using 16S rRNA amplicon metabarcoding, and sequences were analyzed using a combination of mother software and UPARSE.ResultsWe found that the gut microbiome was different between control and AAA mice. The results of correlation analysis between AAA diameter and the gut microbiome as well as LEfSe of the genera Akkermansia, Odoribacter, Helicobacter and Ruminococcus might be important in the progression of AAAs.ConclusionsAAA mice is subjected to gut microbial dysbiosis, and gut microbiota might be a potential target for further investigation.

Highlights

The gut microbiome plays an important role in various cardiovascular diseases, such as atherosclerosis and hypertension, which are associated with abdominal aortic aneurysms (AAAs)
The abdominal aortic aneurysm model was induced by angiotensin Angiotensin II (II) After an overdose of anesthesia, the mice were euthanized
The gut microbial composition changed in the abdominal aortic aneurysm mouse model To explore the gut microbiome in AAA mice, fecal samples were collected before the mice were sacrificed

Summary

Introduction

The gut microbiome plays an important role in various cardiovascular diseases, such as atherosclerosis and hypertension, which are associated with abdominal aortic aneurysms (AAAs). Methods: Here, we used 16S rRNA sequencing to explore gut microbiota in C57BL ApoE−/− mice with AAAs. A mouse model of abdominal aortic aneurysms was induced with angiotensin II (Ang II) (1000 ng/min per kg). Abdominal aortic aneurysm (AAA), a vascular disease with high disability and mortality, is associated with atherosclerosis and hypertension [1]. Recent studies have suggested that gut microbiota probably participate in host inflammation and the formation of atherosclerosis and hypertension [2, 3]. Gut microbiota have been shown to be closely related to systemic inflammation by generating some toxic metabolic factors or by releasing lipopolysaccharides [4].

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