Abstract
Lewis dwarf ( dw/ dw) rats exhibit growth hormone (GH) deficiency and growth retardation linked to a malfunction of GHRH signaling. In this study, GHRH-receptor (GHRH-R) binding and mRNA in the pituitary of adult male dw/ dw and age-matched normal Lewis rats was measured by radioligand binding assay and real-time PCR. Only one of nine pools of dw/ dw pituitary membranes revealed detectable binding of [His 1, 125 I -Tyr 10, Nle 27]hGHRH(1–32) amide ( B max; 4.3 fmol/mg protein). In contrast, GHRH-R binding was 22.4±2.60 fmol/mg protein in normal Lewis rats. mRNA for GHRH-R was detectable in all dw/ dw rat pituitaries examined, averaging 21% that of Lewis rats. Low expression of GHRH-R reflects reduced GHRH-R mRNA as well as a possible reduction in translation of the receptor protein.
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