Abstract
BackgroundThe spontaneous osteoarthritis (OA) in rhesus macaque is similar to OA in human, which maintains an upright body posture and shows very similar biomechanical properties of bones to humans. At present, there is no good treatment for OA. This study aims to explore relationship between OA and intestinal microbiota, and provide a reference for the treatment of clinical OA.ResultsWe collected colonic contents of the 20 rhesus macaque (6–15 years old, female) for intestinal microbiota analysis by metagenomics sequencing, of which 10 were spontaneous OA monkeys and 10 were normal monkeys. Our results showed the diversity of gut microbiota in monkeys with OA was decreased compared to the normal monkeys (p = 0.16). Mollicutes, Tenericutes, Coprobacillus and Faecalitalea may be biomarkers for the monkeys of OA. Lactobacillus found significantly increased in OA monkeys. Prevotella and Ruminococcus were higher in the normal group than OA group. Zinc/manganese transport system permease protein (p = 0.0011) and Cyclopropane-fatty-acyl-phospholipid synthase (p = 0.0012) are a microbiota metabolic pathway related to cartilage production.ConclusionsOur results indicate that the diversity and composition of intestinal microbiota in monkeys with OA are different compared to the normal monkeys. we have found microbes that may be a biomarker for the diagnosis of osteoarthritis. Functional analysis of the microbiota also predicts cartilage damage in the monkeys with osteoarthritis. Non-human primates are closely related to humans, so this study can provide a reference for the development of drugs for the treatment of OA.
Highlights
The spontaneous osteoarthritis (OA) in rhesus macaque is similar to OA in human, which maintains an upright body posture and shows very similar biomechanical properties of bones to humans
Confirmation of spontaneous osteoarthritis models of rhesus macaque The magnetic resonance imaging (MRI) image of the normal monkeys of knee joint shows a clear line of bone growth plate, smooth and boneless woven spine in cartilage, and smooth and even synovial tissue in the joint
Microbiota diversity between OA and normal monkeys Gut microbiota was characterized by metagenomics sequencing
Summary
The spontaneous osteoarthritis (OA) in rhesus macaque is similar to OA in human, which maintains an upright body posture and shows very similar biomechanical properties of bones to humans. There is no good treatment for OA. This study aims to explore relationship between OA and intestinal microbiota, and provide a reference for the treatment of clinical OA. Osteoarthritis (OA) is the most common form of arthritis and the leading cause of chronic disability that mainly affects the knee and hip joints. The widespread existence of osteoarthritis has caused a heavy social and medical burden [1, 2]. Many factors have been discovered to be associated with the occurrence of osteoarthritis including obesity, age, gender, genetic factors and so on [5]. The risk of osteoarthritis related to genetic factors seems to be relatively mild [6]. Non-genetic factors are the main cause of
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