Abstract
Manno-oligosaccharide (MOS) is a prebiotic derived from natural plants or yeasts. Here, we explored the response of intestinal microbiota and epithelial functions after ingestion of MOS in a porcine model. Sixteen pigs were randomly assigned into two treatments and fed with basal or MOS-containing (0.3% MOS) diet for 21 days. Results showed that MOS supplementation increased the cecal acetate content and ileal 16S rRNA gene copies (p < 0.05). Importantly, MOS decreased the abundance of phylum Proteobacteria in cecal digesta (p < 0.05). Moreover, MOS elevated the expression level of SCL5A8 and GPR109A but decreased the expression levels of HDAC1 and TNF-α in the ileal and cecal mucosa (p < 0.05). MOS upregulated the expression levels of tight-junction protein (ZO-1, claudin-1, and occludin) and IGF-1 in the ileum and cecum (p < 0.05). This study presents the alteration of intestinal microbiota composition and intestinal barrier function after MOS administration, and facilitates our understanding of the mechanisms behind the dietary MOS-modulated intestinal microbiota and health.
Highlights
Oligosaccharides are composed of monosaccharide units (2–20) with low molecular weight and low degree of polymerization, which have been looked as prebiotics because of their beneficial effects on intestinal microbiota (Delzenne, 2003; Mussatto and Mancilha, 2007)
This study aimed to investigate the alteration of porcine intestinal microbiota in response to dietary MOS supplementation in weaned pigs
We explored the effect of dietary MOS on intestinal microbiota using a porcine model and found that MOS did not affect the growth performance of weaned pigs
Summary
Oligosaccharides are composed of monosaccharide units (2–20) with low molecular weight and low degree of polymerization, which have been looked as prebiotics because of their beneficial effects on intestinal microbiota (Delzenne, 2003; Mussatto and Mancilha, 2007). Oligosaccharides are resistant to digestion in the upper intestinal tract, but can be fermented by certain microorganisms in large bowel to produce short-chain fatty acids (SCFAs) (Garro et al, 2004; Mussatto and Mancilha, 2007; Quintero-Villegas, 2014). Manno-oligosaccharide (MOS) is a non-digestible oligosaccharide isolated from sugar polymers present in the cell wall of yeast, which is composed of glucose and mannose units through β-1,4 glycosidic bonds (Yu et al, 2020). The health benefits of oligosaccharides have long been appreciated, and a diet containing MOS has been reported to increase the abundance of beneficial bacteria, enhance individual immunity, and maintain the
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