Alteration of payload in extracellular vesicles by crosstalk with mesenchymal stem cells from different origin

Dong Jun Park,Jeong-Eun Park,Tae Hoon Kong,Young Joon Seo

doi:10.1186/s12951-021-00890-9

Abstract

BackgroundThe application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage. We hypothesized that EVs exert different inflammatory effects on one recipient cell, although stem cells of different origins in humans have similar payloads.ResultsHere, the payload of EVs released by crosstalk between MSCs and human middle ear epithelial cells (HMEECs) extracted from adipose tissue, bone marrow and tonsils significantly increased the level of anti-inflammatory factors. EVs derived from the co-culture medium decreased TNF-α, COX-2, IL-1β, and IL-6 levels to approximately zero within 3 h in HMEECs. Expression of miR-638 and amyloid-β A4 precursor protein-binding family A member 2 was analyzed using microarrays and gene ontology analysis, respectively.ConclusionsIn conclusion, stem cells of different origins have different payloads through crosstalk with recipient-specific cells. Inducing specific factors in EVs by co-culture with MSCs could be valuable in regenerative medicine.Graphical abstract

Highlights

The application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage
We evaluated the anti-inflammatory effect of EVs derived from Human mesenchymal stem cell (hMSC) extracted from the adipose tissue, bone marrow, and tonsils
Validation of mesenchymal stem cells derived from adipose tissue, bone marrow, and tonsil using FACS To confirm the effects of MSCs in various locations in the human body, we obtained MSCs from three different human tissues

Summary

Introduction

The application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage. We hypothesized that EVs exert different inflammatory effects on one recipient cell, stem cells of different origins in humans have similar payloads. Researchers have focused on understanding the pathophysiology of OM as a replacement for antibiotic treatment, including the role of inflammatory mediators and ion homeostasis molecules [2]. Restoration of Therapeutics based on stem cell technology, including stem cell-derived EVs, have emerged in recent years and can treat what were otherwise considered incurable diseases in the field of cancer or cell regeneration. It has been found that tumor-derived EVs play a key role in crosstalk between malignant and transformed cells and the immune system and that cancer cells suppress immune surveillance [8]. Stem cell-derived EVs are involved in crosstalk with surrounding cells during development and release their molecules to them [9, 10], and help the survival of surrounding cells through secretion via EVs of inflammatory, differentiation, and proliferation factors [4, 5]

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