Abstract
Introduction: Proteomic studies are becoming popular lately among reproductive biologists in the diagnosis and management of several diseases including male infertility. Objective: To evaluate the level of Neutral-alpha Glucosidase (NAG) in the seminal plasma of men being investigated for infertility in and to correlate its activity with sperm motility. Materials and Methods: Four hundred men age range 23–60 years were consecutively recruited in the study. After physical and clinical evaluation, the semen was collected by self or assisted masturbation and analysis was done according to the World Health Organization guidelines. The semen was then centrifuged and seminal plasma separated for the assay of NAG activity by ELISA technique using reagents supplied by Melson Medical Co, Shangai, China within one week of collection. The participants were categorized in to normozoospermia (191), oligozoospermia (98), severe oligozoospermia (70) and azoospermia (41) based on sperm count. Results: The mean levels of NAG activity decreased with decreasing levels of sperm count with values lowest among azoospermia and highest among normozoospermic subjects. The comparison of NAG activity between the various categories and control subjects was significantly different (P 0.05) when compared with controls. The mean NAG activity level was significantly lower among the asthenozoospermia (P
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