Abstract
The mechanical properties of cells are key to the regulation of cell activity, and hence to the health level of organisms. Here, the morphology and mechanical properties of normal pancreatic cells (HDPE6-C7) and pancreatic cancer cells (AsPC-1, MIA PaCa-2, BxPC-3) were studied by atomic force microscopy. In addition, the mechanical properties of MIA PaCa-2 after treatment with different concentrations of doxorubicin hydrochloride (DOX) were also investigated. The results show the Young's modulus of normal cells is greater than that of three kinds of cancer cells. The Young's modulus of more aggressive cancer cell AsPC-1 is smaller than that of less aggressive cancer cell BxPC-3. In addition, the Young's modulus of MIA PaCa-2 rises with the increasing of DOX concentration. This study may provide a new strategy of detecting cancer, and evaluate the possible interaction of drugs on cells.
Highlights
Pancreatic cancer is a highly malignant tumor [1] with the highest mortality rate and the lowest 5-year survival rate (≤5%) when all stages are combined [2,3]
The doxorubicin hydrochloride (DOX) solution was removed after 4 h and the cells were washed with phosphate-buffered saline (PBS) for three times
The nanostructure and physical properties of normal cells and pancreatic cancer cells (PCCs) were revealed by Atomic force microscopy (AFM)
Summary
Pancreatic cancer is a highly malignant tumor [1] with the highest mortality rate (ca. 100%) and the lowest 5-year survival rate (≤5%) when all stages are combined [2,3]. Pancreatic cancer is a highly malignant tumor [1] with the highest mortality rate 100%) and the lowest 5-year survival rate (≤5%) when all stages are combined [2,3]. Since no significant symptoms appear until the late stage of pancreatic cancer, the development of early diagnosis methods is of importance. The traditional biological methods to research pancreatic cancer are based on molecular genetics and gene signaling. The mechanical properties of cells, which can enable cells to express various biological functions have been ignored [4]. Changes of the physical properties of cells are consid-.
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