Alteration of haematological and biochemical biomarkers after sub-lethal chronic malathion (Elathion®) intoxication in freshwater fish, Labeo rohita (Hamilton, 1822)

Abstract

The present investigation aimed to evaluate the long-term effects of malathion (Elathion®) at two sub-lethal concentrations (0.36 and 1.84 mgL−1) for 45 days after the determination of 96 h-LC50 value (18.35 mgL−1) in a commercially important aquaculture species, Labeo rohita by assaying multiple biomarker approaches. Total erythrocyte count (TEC), and haemoglobulin count (Hb) were found to be decreased while total leucocyte counts (TLC) were increased (p < 0.05) in malathion-intoxicated fish. Malathion exposure significantly reduced (p < 0.05) serum protein levels while significantly increased (p < 0.05) blood glucose levels. RNA activity in muscle was reduced (p < 0.05) while DNA activity increased (p < 0.05) in malathion-intoxicated fish. Acid phosphatase (ACP) activities in the brain; lacate dehydrogenase (LDH) activities in brain and liver were increased (p < 0.05), while alkaline phosphatase (ALP) activities in the brain; succinate dehydrogenase (SDH) activities in the brain, liver and kidney; acetylcholine esterase (AChE) activity in the brain; and ATPase activities in the brain, liver and kidney were reduced (p < 0.05) in comparison to control. Thus, the alteration in studied biomarkers was in a concentation-time dependent manner; however, it was more pronounced at the higher concentration at 45 days of exposure. The alteration in biomarker activity is probably a defensive mechanism/ adaptive response of fish to overcome the stress induced by malathion, which is a novel insight and possible impact on L.rohita. Our findings suggest malathion-induced stress, therefore, the use of malathion needs to be regulated to safeguard aquatic animals including fish and human health.