Alteration of Food intake, Inflammatory Response, and Insulin Resistance by Seaweeds with High‐Fat Diet in C57BL/6N Mice

Abstract

Seaweeds contains various bioactive compounds and health promoting effects. Anti‐inflammatory and anti‐adipogenic effects of seaweeds have been intensively studied, but their mechanism(s) have not been clearly understood yet. In this study, we investigated that effects of four edible seaweeds (Undaria pinnatifida(UP), Laminaria japonica(LJ), Sargassum fulvellum(SF), Hizikia fusiforme(HF)) on alteration of food intake, obesity, insulin resistance, and inflammation by using in vivo and in vitro models. Six week‐old C57BL/6N male mice were fed with either low fat diet, high fat diet (HFD), or HFD+5% of each four edible seaweeds shown above for 16 weeks. Food intake/body weight and fasting blood glucose were measured every other day and every 4 weeks, respectively. Insulin tolerance test (ITT) was performed at 14 week. Additionally, bone‐marrow derived cells from 6 groups were isolated and differentiated to macrophage (BMDM) to evaluate the inflammatory response by LPS challenge. As a result, 5% seaweeds addition to HFD did not alter body weight changes compared to that of HFD fed group. Although food intake data suggested that there was a slight alteration of food intake in the beginning, the changes eventually disappeared. In spite of being obese, LJ contained HFD fed group demonstrated significantly improved insulin sensitivity than HFD only fed group based on ITT. LPS treated BMDM from LJ+HFD mouse expressed significantly less amount of IL‐6 compared to LPS treated BMDM from HFD group. In conclusion, LJ among 4 types of edible seaweeds demonstrated to have potential anti‐diabetic and anti‐inflammatory effects in diet induced obese model. This work was supported by NRF‐2013R1A1A1057573