Abstract
Abstract One of the remarkable features of bacterial species is their capacity for rapid growth when the appropriate environmental condition for growth is provided. Some bacteria, during their growth period, encounter stress factors in their natural environments, such as limitation in growth bioavailability, heat shock, heavy metal, etc. One stress factor not studied is the effect of magnetic Fe3O4 nanoparticles on bacterial growth rate. The effect of magnetic Fe3O4 nanoparticles on the protein profiles of genetically engineered bacterial strain Pseudomonas aeruginosa (PTSOX4), a strain with biological desulfurization characteristic, was investigated. The magnetic Fe3O4 nanoparticles were synthesized using co-sedimentation method, and their morphology was observed by scanning electron microscopy (SEM). The topography of magnetic Fe3O4 nanoparticles was detected by X-ray diffraction, and the average nanoparticle size measured was 40 to 50 nm. The bacterial cells were coated with magnetic nanoparticles, and the SEM electrographs of the bacterial cells indicated that the nanoparticles were uniformly coated on the cell surface. Proteins from both uncoated and coated bacterial cells were extracted by sonication and subjected to two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. Some novel protein bands appeared in the protein profiles of coated bacterial cells; however, some protein bands disappeared. The two-dimensional gel electrophoresis results highlighted the presence of two different polypeptide groups, with molecular weights of 30 to 56 kDa and 56 to 65 kDa.
Highlights
The combination of nanotechnology and biological science has been created a new path for the researchers to exploit in various biological fields
Tauc plot is shown in Fig. 1b and the band-gap energy was calculated as 2.8 eV for zinc oxide nanoparticles (ZnO NPs) synthesized using B. tomentosa leaf extract which was in good agreement with the result reported by Khuili et al [17]
Zinc oxide nanoparticles were successfully synthesized by biogenic route using B. tomentosa leaf extract
Summary
The combination of nanotechnology and biological science has been created a new path for the researchers to exploit in various biological fields. The alternative method to synthesize nanoparticles in an eco-friendly, biocompatible, safe, and cost-effective is green approach, which allows large-scale production of nanoparticles through bacteria, fungi, algae, and plants Different plant parts such as root, stem, leaf, flowers, and fruits are rich in phytochemical substances and act as a stabilizing and reducing agent in the production of nanoparticles [8,9,10]. Tauc plot (αhν versus hν) is shown in Fig. 1b and the band-gap energy was calculated as 2.8 eV for ZnO NPs synthesized using B. tomentosa leaf extract which was in good agreement with the result reported by Khuili et al [17]. Due to the presence of alkaloids, terpenoids, flavonoids, tannins, carbohydrates, sterols, saponins, proteins, and amino acids in B. tomentosa leaf extract showed potential bioreducing activity and bactericidal activity against the tested bacteria which could be useful for biomedical applications
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