Abstract
The intra-plant distribution of 15N in common bean, cowpea and soybean having different levels of responses to shading and N2-fi xing ability were analyzed under shaded and non-shaded conditions. Maize was used as a reference (non N2-fixing) plant. Seedlings were grown in pot soils for 3 weeks then transferred to shaded (55% of control) and non-shaded (control) conditions in a greenhouse, and sampled at 13 days and 24 days after shading. The proportion of plant N derived from N2-fixation (%Ndfa) estimated by the natural 15N abundance method was higher in cowpea and soybean (74.91%) than in common bean (37.38%). Shade treatment reduced %Ndfa signifi cantly in cowpea and soybean. The difference in δ15N between shoot and root (Δδ 15Ns-r) was the highest in maize followed by common bean, cowpea and soybean. Shading increased Δδ 15Ns-r in each legume species, particularly in cowpea and soybean. A signifi cant negative correlation was found between Δδ 15Ns-r and %Ndfa in all legumes at both sampling dates (R2 = 0.67.0.96, P<0.1). The slope and Y-intercept of the regression line was similar at the sampling dates, but varied with the species. The slope was –0.05 in cowpea, –0.06 in common bean, and –0.11 in soybean. The Δδ 15Ns-r value estimated by extrapolation of the regression line was 2.9, 2.5 and 8.6%. at 0 %Ndfa, and –3.2, –2.8 and –2.6%. at 100 %Ndfa, in common bean, cowpea and soybean, respectively. The consistent relationships between Δδ 15Ns-r and %Ndfa found among legume species suggest that Δδ 15Ns-r could be used as a parameter for estimating %Ndfa without using a reference plant, although the component of regression line was characteristically different among legume species.
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