Alteration in dopaminergic neurotransmission following exposure to immobilization stress on the neurobehavioral toxicity of lambda-cyhalothrin in rats

Abstract

Event Abstract Back to Event Alteration in dopaminergic neurotransmission following exposure to immobilization stress on the neurobehavioral toxicity of lambda-cyhalothrin in rats Rajendra K. Shukla1, 2*, Richa Gupta1, Lalit P. Chandravanshi1, Yogesh K. Dhuriya1, Pranay Srivastava1, Mohammad H. Siddiqui3, Ajay Kumar2, Aditya B. Pant1 and Vimay K. Khanna1 1 CSIR-Indian Institute of Toxicology Research, Developmental Toxicology Laboratory, System Toxicology and Health Risk Assessment Group , India 2 Integral University, Department of Biochemistry, Integral Institute of Medical Sciences & Research, India 3 Integral University, Department of Bioengineering, Faculty of Engineering, India Impact of stress in exacerbating the toxicity of environmental chemicals has been suggested whereas the effect and mechanism associated are not clearly understood. Therefore the present study has been carried out to investigate the influence of immobilization stress (IMS), a psychological stressor on the neurobehavioral toxicity of lambda-cyhalothrin (LCT), a new generation type-II synthetic pyrethroid with extensive applications in outdoor and indoor including public health programmes. Rats subjected to IMS (15 min/day) for 28 days or exposed to LCT (3.0 mg/kg body weight, p.o.) for 3 days (day 26, 27 and 28) had no significant effect on DA-D2 receptors, neurotransmitter levels of DA, DOPAC and HVA and immunoreactivity of tyrosine hydroxylase in corpus striatum, plasma corticosterone levels, blood-brain barrier permeability associated with motor activity as compared to controls. Marginal changes were observed in the activity of mitochondrial complexes, expression of selective anti-apoptotic (Bcl2) and pro-apoptotic (Bax, Caspase-3) proteins in the corpus striatum of rats exposed to either IMS or those exposed to LCT as compared to controls. Pre-exposure to IMS for 28 days followed by LCT exposure for 3 days in rats resulted in increased plasma corticosterone levels, blood-brain barrier permeability and decrease motor activity, DA-D2 receptors, expression of tyrosine hydroxylase and alteration in levels of DA, DOPAC and HVA in corpus striatum as compared to rats exposed to IMS or LCT alone. It was interesting to note that pre-exposure to IMS followed by LCT treatment also caused a marked decrease in mitochondrial dysfunctions associated with decreased the expression of anti-apoptotic while pro-apoptotic and stress marker proteins was enhanced in corpus striatum of these rats as compared to those exposed to IMS or LCT alone. The results exhibit that psychological stresses contribute in the LCT induced dopaminergic alterations associated with mitochondrial dysfunctions. Keywords: Dopamine, neurotransmission, Neurotoxicity, in vivo, Neurobehaviour Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: O03: Postgraduate Travel Awardees Oral Session 3 Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Shukla RK, Gupta R, Chandravanshi LP, Dhuriya YK, Srivastava P, Siddiqui MH, Kumar A, Pant AB and Khanna VK (2016). Alteration in dopaminergic neurotransmission following exposure to immobilization stress on the neurobehavioral toxicity of lambda-cyhalothrin in rats. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00094 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Mr. Rajendra K Shukla, CSIR-Indian Institute of Toxicology Research, Developmental Toxicology Laboratory, System Toxicology and Health Risk Assessment Group, Lucknow, Uttar Pradesh, India, razshukla@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Rajendra K Shukla Richa Gupta Lalit P Chandravanshi Yogesh K Dhuriya Pranay Srivastava Mohammad H Siddiqui Ajay Kumar Aditya B Pant Vimay K Khanna Google Rajendra K Shukla Richa Gupta Lalit P Chandravanshi Yogesh K Dhuriya Pranay Srivastava Mohammad H Siddiqui Ajay Kumar Aditya B Pant Vimay K Khanna Google Scholar Rajendra K Shukla Richa Gupta Lalit P Chandravanshi Yogesh K Dhuriya Pranay Srivastava Mohammad H Siddiqui Ajay Kumar Aditya B Pant Vimay K Khanna PubMed Rajendra K Shukla Richa Gupta Lalit P Chandravanshi Yogesh K Dhuriya Pranay Srivastava Mohammad H Siddiqui Ajay Kumar Aditya B Pant Vimay K Khanna Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.