Abstract
Analysis of the frequency and PCR-quantifiable abundance of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV) DNA in multiple biological replicates of cells from dissociated randomly distributed human trigeminal ganglia (TG) of four subjects revealed an increase in both parameters and in both viruses during 5days of culture, with no further change by 10days. Dissociated TG provides a platform to analyze initiation of latent virus DNA replication within 5days of culture.
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