Abstract
Using a titration procedure, we measured the proportion of alpha-fetoprotein (AFP) and albumin mRNA in normal, regenerating, and preneoplastic rat livers. AFP mRNA constitutes approximately 0.006% of the polysomal polyadenylated RNA of normal livers and this proportion increases only slightly before the onset of DNA synthesis in liver regeneration induced by partial hepatectomy or CCl4 injury. In either model of liver regeneration, the proportion of AFP mRNA in polysomal RNA is highest approximately 24 h after the peak of DNA synthesis. The increase in the proportion of AFP mRNA in polysomal RNA is relatively small during liver regeneration (2-4-fold) but is larger (30-50-fold) in preneoplastic livers of rats fed a choline-deficient diet containing 0.1% ethionine. In contrast to those changes in AFP mRNA, albumin mRNA levels remain unchanged during liver regeneration and double in preneoplastic livers. Our results indicate that the concept of "retrodifferentiation" as it applies to liver regeneration and certain types of hepatic neoplasia needs reevaluation.
Highlights
MRNA constitutes approximately 0.006%of the poly- adult organ [1,3,4,5]
We investigated if there is a preferential increase in hepatic AFP mRNA and a corresponding decrease in ALB mRNA during liver regeneration and hepatocarcinogenesis and whether major changes in the expression of these genes precede the peak of DNA synthesis during liver regeneration
Those changes in AFP mRNA,albuminmRNA levels remain unchanged during liver regeneration and double in preneoplastic liverOsu. r results indicate that the MATERIALS AND METHODS~
Summary
The concept of retrodifferentiation implies that the pattern buffer (0.24 M sodium phosphate buffer (pH 6.9), 1.0 mM disodium of gene expression of differentiated cells resembles that of the corresponding embryonic cells [1,2]. Full size photocopies ribonucleotides (deoxyribonucleotides) in moles/liter multiplied by are included in the microfilm edition of the Journal that is the incubation time in seconds.
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