Abstract
[3H] Rauwolscine ([3H]Ra), a selective ligand for the alpha2 adrenergic receptor, was used to identify and characterize alpha2 adrenergic receptors in prostate glands of men with benign prostatic hyperplasia. Specific binding of [3H]Ra to prostatic tissue homogenates was rapid and readily reversible by addition of excess unlabelled phentolamine. Scatchard analysis of saturation experiments demonstrates a single, saturable class of high affinity binding sites (Bmax = 0.31 ± 0.04 fmol./μg. DNA, Kd = 0.9 ± 0.11nM.). The relative potency of alpha adrenergic drugs (clonidine, alphamethylnorepinephrine and prazosin) in competing for [3H]Ra binding sites was consistent with the order predicted for an alpha2 subtype. The role of alpha2 adrenergic receptors in normal prostatic function and in men with bladder outlet obstruction secondary to BPH requires further investigation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
