Abstract
BackgroundThe effects of α-tocopherol on intracellular Ca2+ intensity in semen cryopreservation by regulate intracellular Ca2+ intensity have not been reported yet.ObjectiveThe research was conducted to evaluate the effect of supplementation α- tocopherol into egg yolk skim milk extender on sperm quality and intracellular Ca2+ intensity.MethodsSemen samples were collected and supplemented with respectively 0mM (P0); 0.5mM (P1); 1mM (P2); 1.5mM (P3) and 2mM (P4) α-tocopherol in extender before cryopreservation processes. Post-thawing sperm was evaluated for motility, viability, and abnormality using Phase Contrast Microscope (200x) with eosin-nigrosine staining, and intracellular Ca2+ intensity of the best result dose was evaluated using Confocal Laser Scan Microscope (400x) with Fluo-3 Staining.ResultsThe results showed there was a significant difference (P≤0.05) in sperm motility and viability between P0; P1 with P2; P3; P4. The Motility and viability between groups P0; P1 and P3; P4 showed no significant difference (P≥0.05), while P2 with P3; P4 showed significant difference (P≤0.05). There was a significant difference (P≤0.05) in sperm abnormality of P0; P1 with P2; P3; P4. The abnormality between P0; P1 and P2; P3 showed no significant difference (P≥0.05), while P2; P3 showed a significant difference with P4 (P≤0.05). The best result in sperm quality was supplementation with 1.5mM α-tocopherol. Ca2+ intracellular intensity: 142.76± 21.8 au (P0) and 176.06±61.43 au (P3).ConclusionsIt was concluded that 1.5mM α-tocopherol is the best dose to improve sperm quality by regulating intracellular Ca2+ intensity on Simmental bull cattle.
Highlights
Artificial insemination has been implemented to improve livestock production in agricultural practices.[1]
1.5mM α-tocopherol is the best dose to motility, viability and abnormality morphol- Each ejaculates was extended using Egg improve sperm quality by regulating intra- ogy were evaluated after freezing- yolk Skim Milk, semen was supplemented cellular Ca2+ intensity on Simmental bull thawing
Article straw was placed above liquid nitrogen (LN2) Sperm quality cant difference (P≤0.05)
Summary
Artificial insemination has been implemented to improve livestock production in agricultural practices.[1] Several factors that affect sperm motility during the artificial insemination is semen quality, including the Ratnani H,1 Suprayogi T.W.,1 Sardjito T,1 Susilowati S,1 Azura S2. Contributions: RH, SS, data analysis, manuscript writing, and references search; STW, Surabaya, Indonesia. Semen cryopreservation may induce lipid ST, data collecting and analysis; AH, data colperoxidation that leads an excessive pro- lecting. Abstract duction of Reactive Oxygen Species (ROS), which reduce the motility of sperm.[4] Cryopreservation may alter the mem-. The effects of α-toco- brane plasm permeability of spermatozoa pherol on intracellular Ca2+ intensity in against ion Ca2+.
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