Abstract
The plant Psychotria malayana Jack belongs to the Rubiaceae family and is known in Malaysia as “meroyan sakat/salung”. A rapid analytical technique to facilitate the evaluation of the P. malayana leaves’ quality has not been well-established yet. This work aimed therefore to develop a validated analytical technique in order to predict the alpha-glucosidase inhibitory action (AGI) of P. malayana leaves, applying a Fourier Transform Infrared Spectroscopy (FTIR) fingerprint and utilizing an orthogonal partial least square (OPLS). The dried leaf extracts were prepared by sonication of different ratios of methanol-water solvent (0, 25, 50, 75, and 100% v/v) prior to the assessment of alpha-glucosidase inhibition (AGI) and the following infrared spectroscopy. The correlation between the biological activity and the spectral data was evaluated using multivariate data analysis (MVDA). The 100% methanol extract possessed the highest inhibitory activity against the alpha-glucosidase (IC50 2.83 ± 0.32 μg/mL). Different bioactive functional groups, including hydroxyl (O-H), alkenyl (C=C), methylene (C-H), carbonyl (C=O), and secondary amine (N-H) groups, were detected by the multivariate analysis. These functional groups actively induced the alpha-glucosidase inhibition effect. This finding demonstrated the spectrum profile of the FTIR for the natural herb P. malayana Jack, further confirming its medicinal value. The developed validated model can be used to predict the AGI of P. malayana, which will be useful as a tool in the plant’s quality control.
Highlights
The long-term metabolic disease produced by hyperglycemia is known as diabetes mellitus (DM), referring to a condition where a consistently high blood sugar level leads to an imbalance of tissueMolecules 2020, 25, 4161; doi:10.3390/molecules25184161 www.mdpi.com/journal/moleculesMolecules 2020, 25, 4161 homeostasis
T2DM represents most of the cases worldwide [1]
The 75% solvent ratio of methanol-water extracts of P. malayana resulted in the highest yield (35.19 ± 1.11%)
Summary
The long-term metabolic disease produced by hyperglycemia is known as diabetes mellitus (DM), referring to a condition where a consistently high blood sugar level leads to an imbalance of tissueMolecules 2020, 25, 4161; doi:10.3390/molecules25184161 www.mdpi.com/journal/moleculesMolecules 2020, 25, 4161 homeostasis. The long-term metabolic disease produced by hyperglycemia is known as diabetes mellitus (DM), referring to a condition where a consistently high blood sugar level leads to an imbalance of tissue. Various complications may develop if diabetes is not well managed; for instance, it can cause cardiovascular diseases and stroke [1]. The World Health Organization (WHO) has categorized three main variants of diabetes mellitus and identified it as a heterogeneous metabolic disease with high blood sugar [2]: type-1, insulin-dependent diabetes (T1DM); type-2, non-insulin-dependent diabetes (T2DM); and a third, gestational diabetes mellitus (GDM). T2DM predominantly affects obese adolescent children and has been observed more in older individuals [2]. T2DM represents most of the cases worldwide [1].
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