Abstract
Alpha‐1‐fetoprotein (AFP) was determined by different immunochemical techniques in the serum of a patient with liver cirrhosis. Although a precipitation line was observed against anti‐AFP antiserum, using bidimensional immunodiffusion, immunoelectrophoresis, countercurrent electrophoresis and rocket electrophoresis, this precipitation line however showed a reaction of non‐identity with a positive AFP control. Several different commercial anti‐AFP antisera gave the same false‐positive result. Analysis of the patient's serum revealed the falsely positive reacting component to be of IgM‐like nature, having a cathodal mobility. These findings are discussed in the light of the determination of slightly elevated AFP levels by a method such as the radioimmunoassay, not allowing identification by means of identity with control specimen. The consequence of our results is also discussed with respect to normal serum AFP.The described non‐AFP activity was detected regularly in sera taken over the period 1969–1976.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
