Abstract
Objective To investigate the immune functions of dentritic cells (DCs) transduced with Ad-IL-18, Ad-AFP in vitro, compare their antiviral and antitumor effects with those of Ad-IL-18/AdAFP DCs and Ad-AFP DCs.Methods Peripheral blood mononuclear cells (PBMC) from HCC patients were isolated by blood separator.PBMC were cultured in vitro for one week to induce DCs.After transfection with Ad-IL-18 and Ad-AFP (IL-18/AFP-DC) ,the stimulated lymphocytes were tested by cytotoxicity capacity assay.Results The results showed that DCs could infect and express relevant genes.The expression of CD11c (84.3%) ,CD1a (73.4%) ,CD80 (89.5%) ,CD86 (87.9%) and HLA-DR (91.7%) on adenovirus transduced DCs were up-regulated.The cytotoxicity mediated by IL-18/AFP-DC-T was high-er than that mediated by DC-T and T,and that mediated by IL-18/AFP-DC-T was higher than that mediated by IL-18-DC-T and AFP-DC-T.Conclusion DCs co-infected with these vectors can induce antitumor CTL response in vitro.And Ad-AFP and Ad-IL-18 transfected DCs could induce stronger CTL response against HepG2 than AFP gene-transfected DCs. Key words: Carcinoma,hepatocellular; Dendritic cells; Cytotoxic T lymphocytes; Immunotherapy
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