Abstract
Extracts of hulls from developing Brassica campestris and Brassica napus seeds were examined for α-amylase activity. The enzyme was compared with α-amylase from barley pericarp and germinated barley seeds, using isoelectric focusing. Enzymes of similar isoelectric point (pI) were present in B. campestris, B. napus, and barley pericarp. Additional bands of activity were present in B. napus. The major α-amylase component of germinated barley has a high pI, but no corresponding isozyme was detected in the other three samples. The increase in total activity of α-amylase in Brassica seed hulls coincided with the increase in starch granule size in the epidermal cell layer, with maximum activity approximately 16 days after pollination.
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