Alpha amylase and angiotensin converting enzyme inhibitory potential of aqueous extract of Azanza garckeana fruit

Abstract

Diabetes mellitus and hypertension are common diseases affecting a lot of people. Alpha amylase and angiotensin-converting enzyme (ACE) inhibitors are used to treat type II diabetes and hypertension respectively. This study investigated the alpha amylase and ACE inhibitory potential of Azanza garckeana fruits. Phytochemical screening, α-amylase and ACE inhibitory potential of the aqueous extract of A. garckeana fruit was determined using standard procedures. The mode of inhibition of α-amylase by A. garckeana fruit was determined from the Lineweaver-Burk plot. Alkaloids, flavonoids, anthraquinones, steroids, tannins, phenols and terpenoids were present in the aqueous extract of A. garkeana fruit. The percent inhibition of α-amylase was greater than 50%. The IC50 values were 2.6 ± 0.02 and 0.04 ± 0.09 for the extract and acarbose (standard drug) respectively. The Lineweaver-Burk plot showed that extract Vmax did not change when compared to the no inhibitor (no extract) but the km increased. The percent inhibition of ACE by A. garckeana was also greater than 50%. Its IC50 was 0.625 ± 0.03 while that of the standard drug (captopril) was 0.875 ± 0.07. Thus A. garckeana inhibited α-amylase and ACE and can be used to treat type II diabetes and hypertension. It is a competitive inhibitor of α-amylase.