Abstract
A novel strategy for the preparation of amperometric immunosensor for rapid determination of α-1-fetoprotein (AFP) in human serum has been developed. TiO 2 nanoparticles (NPs) were prepared by solvothermal reaction using TiCl 4 as raw materials and the mixture of ionic liquids and doubly distilled water as solvent. α-1-fetoprotein antibody (AFP Ab) was mixed with TiO 2 NPs/chitsotan (CHIT) solution and immobilized onto the surface of a glassy carbon electrode. AFP (Ab) functionalized Au NPs were used as catalytic labels for the amperometric detection of AFP by means of the electrocatalyzed reduction of Au NPs to H 2O 2. The electrochemical behavior of the immunosensor was studied. Other experimental conditions such as pH, immunoreactions temperature and time were also studied. The prepared immunosensor offers an excellent amperometric response for AFP ranging from 1.0 to 160.0 ng/mL with a detection limit of 0.1 ng/mL. The result shows that the immunosensor displays rapid response, high sensitivity, good reproducibility and favorable stability.
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