Abstract
This study aimed to evaluate the extract of Aloe vera (AV) associated or not with 10% Dimethylsulfoxide (DMSO) in cryopreservation of tambaqui semen. For the formation of the pools (n= 14), 30 males were hormonally induced twice. Each pool had the objective motility, curvilinear velocity, straight-line velocity, average path velocity and morphology analyzed before and after cryopreservation of semen. The means for cryopreservation were constituted of Powder Coconut Water-104 diluent added DMSO and/or AV (5 or 10%). After cryopreservation, motility, velocities and morphology were reduced significantly when compared to fresh semen. For sperm motility the best treatment was that using only DMSO (20,86±8,31) and DMSO + 5% AV (15.71±9.77). For the velocities, the worse treatment was DMSO+10% AV. Treatment with only the addition of DMSO had a significantly higher effect than others on percentage of morphologically normal sperm. The mean correlation found was between motilityand the rate of morphologically normal sperm (r = 0.687). In conclusion, the addition of AV does not provide greater protection for spermatozoa during cryopreservation.
Highlights
Agradeço ao Departamento Nacional de Obras Contra as Secas (DNOCS) por ceder os animais e a estrutura para a realização do experimento; à empresa a Água de Coco em Pó (ACP) Biotecnologia por fornecer o meio para criopreservação; à Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP) pela concessão da bolsa de estudos; e à Financiadora de Estudos e Projetos (FINEP) pelo suporte financeiro para a realização dos experimentos
This study aimed to evaluate the extract of Aloe vera (AV) associated or not with 10% Dimethylsulfoxide (DMSO) in cryopreservation of tambaqui semen
The means for cryopreservation were constituted of Powder Coconut Water-104 diluent added DMSO and/or AV (5 or 10%)
Summary
Agradeço ao Departamento Nacional de Obras Contra as Secas (DNOCS) por ceder os animais e a estrutura para a realização do experimento; à empresa ACP Biotecnologia por fornecer o meio para criopreservação; à Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP) pela concessão da bolsa de estudos; e à Financiadora de Estudos e Projetos (FINEP) pelo suporte financeiro para a realização dos experimentos. Aloe vera na criopreservação do sêmen de tambaqui (Colossoma macropomum) Estudos de criopreservação de sêmen utilizando a Água de Coco em Pó (ACP) já foram testados com sucesso em diferentes espécies de peixes, como o Prochilodus lineatus (Viveiros et al, 2010), Colossoma macropomum (Leite et al, 2011) e Piaractus brachypomus (Melo, 2010b), assim como o crioprotetor interno Dimetilsufóxido (DMSO) (Salmito-Vanderley et al, 2012).
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