Abstract
In this study, we assessed the cytotoxic effect of 1,3-diphenyl-2-allyl-1,3-propanedione (DPAP) on B16F10 and Tm5 melanoma cells and investigated its interaction with DNA (deoxyribonucleic acid) using optical tweezers. The compound showed to be effective against B16F10 cells with selectivity index (SI) of 7.92 and interaction to the outer surface of the double helix, probably at the minor groove of DNA. This likely induced a bending deformation of the polymer chain, decreasing persistence length, and indicated that each DPAP molecule occupies a single base pair upon binding to the double helix.
Highlights
Melanocytic neoplasms range from benign lesions, known as melanocytic nevi, to malignant lesions, termed melanomas
On the basis of the results discussed above, we propose the following binding mechanism for the DNA-DPAP interaction: the drug binds to the outer surface of the double helix, probably at the minor groove, as its molecular weight is relatively small
We tested the antineoplastic efficiency of a dibenzoylmethane derivative, DPAP, against B16F10 and Tm5 melanoma cells
Summary
Melanocytic neoplasms range from benign lesions, known as melanocytic nevi, to malignant lesions, termed melanomas. We were able to measure the force-extension curve of the bare DNA molecule and of its complexes formed with DPAP at various different concentrations of the compound.
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