Abstract
Objective To study the protective effect and mechanism of all-transretinoic acid (ATRA) on hypoxia/re-oxygenation induced RAW264.7 murine macrophages injury. Methods RAW264.7 murine macrophages were divided into control, model and ATRA groups. The cell morphological changes of each group were observed directly under inverted microscope. M1 marker inducible nitric oxide synthase (iNOS), M2 marker cluster of differentiation (CD) 206, and peroxisome proliferator activated receptor-γ (PPAR-γ ) were separately examined by RT-PCR, Western blot analysis and immunofluorescence staining. Results In ATRA group, the cell number increased significantly when compared with model group, and the cell physiological status were much better. Compared to model group, the immunofluorescence, expression level of mRNA, and protein level of iNOS significantly reduced in ATRA group (P<0.01). In ATRA group, the immunofluorescence, expression level of mRNA and protein of CD206 and PPAR-γ dramatically increased compared with Model group (P<0.01). Conclusion ATRA attenuated hypoxia/re-oxygenation injury by altering macrophages phenotype switch through down regulation of iNOS and up regulation of CD206 and PPAR-γ. Key words: All-transretinoic acid; Hypoxia/re-oxygenation injury; Macrophages; Peroxisome proliferator activated receptor-γ
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