Alloxan 糖尿病ラットの歯周組織におよぼす局所的刺激の影響

Abstract

The effects of diabetes and local irritation on the periodontal tissues of rats were investigated histologically and histochemically. Diabetes was induced by an intraperitoneal injection of alloxan (18mg/100g of body weight). The alloxan diabetic rats were divided into two groups, one group that received no insulin injections (Non-Insulin group) and the other group that received 0.6I.U./kg of insulin daily (Insulin group). The control group received neither alloxan nor insulin injections (Control group). In the animals of all three groups, a rubber ring was inserted as a local irritating factor into the gingival crevice of the right maxillary first molar, and then removed 12 hours later. The left maxillary first molar did not receive such a treatment and was used as a control tooth. The animals of three groups were killed immediately after removal of the rubber, and 1, 3, 5 and 7 days after removal. The periodontal tissues of the animals were examined histologically and histochemically.1. When the local irritation was absent, the destructive changes (loss of gingival epithelium and connective tissue, inflammatory infiltration, pocket formation, and disorganization of the periodontal fibers) in the periodontal tissues of Non-Insulin and Insulin groups did not differ from those of the Control group. When the local irritation was present, however, the changes were severer in Non-Insulin and Insulin groups than in the Control group.2. In the Control group, the periodontal destruction was gradually restored after removal of the rubber, and the periodontal tissues appeared almost normal at 7 days.3. In Non-Insulin and Insulin groups, the periodontal destruction was still prominent at 1 and 3 days. Both groups, however, showed the repair of the periodontal tissues at 5 days, and it was more marked in the Insulin group.4. In the Non-Insulin group, the periodontal destruction was relatively well restored but still present at 7 days. The periodontal tissues were similar to those of the Insulin group at 5 days.5. In the Insulin group, the periodontal destruction almost disappereared at 7 days, and the periodontal tissues were almost the same with those of the Control group at 5 days.