Abstract
Anti- Salmonella abortus-equi antibodies of restricted heterogeneity produced in large amounts by a rabbit have been fractionated to show individual spectrotypes simple enough to give, in most of the cases, a single focused protein band. Idiotypic and allotypic specificities of these antibodies were studied. With two anti-idiotypic sera which recognized the same idiotypes no difference was found in the idiotypic specificities of the antibodies from nine fractions with individual spectrotypes ranging from pH 5.5 to pH 6.5. The rabbit which developed this antibody response was heterozygous for the b series allotypic specificities. The two allotypic specificities encoded by the related allelic genes were revealed in all the antibody fractions despite the fact that antibodies of most of these fractions exhibited only a single focused IgG band upon 1EF analysis. Presumably the amino acid substitutions related to the b series allotypic specificities are not involved in the electric charge of the entire molecule. Therefore spectrotypes showing a single focused protein band are not always indicative of homogeneity for a product with such an electrophoretic behavior.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
