Abstract
Three out of five rabbits subjected to successive irradiations and immunized against Salmonella abortus-equi produced IgG which, transiently, were not precipitated by anti-a3 anti-allotypic sera, although they carried all the determinants of the a3 allotypic pattern. As a3 IgG from normal rabbits are precipitated by anti-a3 sera, it appeared that the molecular distribution of the a3 allotypic determinants was different on the IgG produced by these irradiated rabbits compared to IgG produced by normal rabbits. After a second irradiation, one of these rabbits produced a high level (50 mg per ml) of anti-Salmonella antibodies of restricted heterogeneity. An anti-idioytpic serum prepared against anti-Salmonella antibodies produced by this rabbit after the first irradiation precipitated the idiotypes it recognized in the serum collected after the first irradiation, while it did not precipitate the idiotypes it recognized in the serum collected after the second irradiation, although they carried all the determinants of the idiotypes of the serum collected after the first irradiation. That probably means that there is a different molecular distribution of the idiotypic determinants between antibodies produced after the first irradiation and antibodies produced after the second irradiation. An antiidiotypic serum prepared against anti-Salmonella antibodies produced after the second irradiation did not distinguish by precipitation in gel medium or by radioimmunoassay between the idiotypes it recognized in antibodies produced after the first irradiation and those in antibodies produced after the second irradiation. The idiotypic similarity thus detected, and the fact that we did not detect unexpected allotypes, is in better agreement with the expression of the potentiality of radiation-resistant cells than with the expression of new antibody producing cell clones arising from virgin stem cells.
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