Abstract
ABSTRACT The objective in this study was to evaluate the clinic effect of applying allogenic platelet-rich plasma (PRP) heated or not, for treating cornea ulcers, including the dosage of PDGF-BB in the cornea. The ulcers were induced, standardizing the left eye from 81 rats (Ratus norvegicus, albinus variety), assigned randomly into three groups (N=27): control group (CG) which did not receive any topic treatment; heated PRP group (GA) and PRP group (GP), which received topical treatment every eight hours for five days. Each group underwent evaluation at 24 hours (M1), three days (M3) and five days (M5). The clinical exam evaluated the opacity, vascularization and corneal repair. The corneal PDGF-BB was dosed through the ELISA method. The corneal opacity was decreased in PRP-treated animals (GA and GP) and corneal repair time reduced when compared to CG at M1 and M5. Furthermore, GP showed greater vascularization at M3 compared to M1. Applied allogenic PRP eye drops, heated or not, speed up corneal healing, and reduce corneal repair time. However, the corneal PDGF concentration was not altered in any of the treatments.
Highlights
Ulcerative keratitis is the most frequent ocular affliction in animals and occurs when the epithelium and a significant amount of theAmong the main growth factors in active corneal cicatrization is the platelet-derived growth factor (PDGF), which is necessary to modulate the proliferation, migration and differentiation of corneal and other cells (Singh et al, 2014)
It is known that platelet-rich plasma (PRP) has provided excellent results, accelerating the healing when applied in ophthalmology (Alió et al, 2007a, 2007b; Geremicca et al, 2010; Panda et al, 2012; Alió et al, 2013)
It was concluded that the use of heated PRP is an option to treat ocular surface diseases in patients that have the autoimmune system as the basic etiology, since heating decreased the immunoglobulin E (IgE) levels and the supplemental system but maintained most of the proteins and other components involved in the cicatrization process
Summary
Ulcerative keratitis is the most frequent ocular affliction in animals and occurs when the epithelium and a significant amount of theAmong the main growth factors in active corneal cicatrization is the platelet-derived growth factor (PDGF), which is necessary to modulate the proliferation, migration and differentiation of corneal and other cells (Singh et al, 2014). Studies that evaluated blood-based derivatives have shown that they are effective and safe in the treatment of many diseases of the ocular surface (Anitua, 2015). The derivatives found include platelet-rich plasma (PRP) characterized by high concentrations of platelets and growth factors, which are released by alpha granules from the platelets (Anitua et al, 2004). The use of PRP against ocular surface diseases has been often studied and applied. The epidermal growth factor (EGF), proteins in the supplement system and immunoglobulin E (IgE), presented significant decreases. It was concluded that the use of heated PRP is an option to treat ocular surface diseases in patients that have the autoimmune system as the basic etiology, since heating decreased the IgE levels and the supplemental system but maintained most of the proteins and other components involved in the cicatrization process. The author suggested in vivo studies to evaluate the efficiency and potential of the heated PRP
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