Abstract
BackgroundAllium hookeri (AH) is widely consumed as a vegetable and herbal medicine in southeastern Asia. AH has been reported antioxidant, antimicrobial, improvement of bone health and antidiabetic effects. In the present study, we investigated the inhibitory effect of a methanol extract of AH root (AHE) on inflammatory response in lipopolysaccharide (LPS)-induced RAW264.7 cells.MethodsInitially, characterization of organic sulfur compounds in AHE was determined using high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). Cells were incubated with LPS and AHE for 24 h. The productions of nitric oxide (NO), reactive oxygen species (ROS), and inflammation-related cytokines were examined. Gene and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were assessed by polymerase chain reaction and Western blotting. Key factor, nuclear factor kappa B (NF-κB) was also determined.ResultsAHE contained organosulfur compounds such as alliin and S-allylcysteine by HPLC-ESI-MS. AHE significantly inhibited NO, ROS, and cytokines production in LPS-induced RAW264.7 cells. In addition, AHE treatment inhibited iNOS and COX-2 mRNA and protein levels, leading to a decrease in iNOS-derived NO level. Furthermore, NF-κB activation was, at least in part, suppressed by AHE treatment.ConclusionOur data suggest that AHE treatment inhibits the inflammation condition through suppression of iNOS and COX-2 expression via NF-κB down-regulation.
Highlights
Allium hookeri (AH) is widely consumed as a vegetable and herbal medicine in southeastern Asia
We demonstrated that methanol extract of AH root (AHE) exhibited the anti-inflammatory effect LPS-induced RAW264.7 cells [24]
The primary antibodies against inducible nitric oxide synthesis (iNOS) (#2982), COX-2 (#4842), Nuclear factor kappa B (NF-κB) (p65) (#8242) and IκBα (#4812) were obtained from Cell Signaling (Cell Signaling Tech., Beverly, MA, USA)
Summary
Allium hookeri (AH) is widely consumed as a vegetable and herbal medicine in southeastern Asia. We investigated the inhibitory effect of a methanol extract of AH root (AHE) on inflammatory response in lipopolysaccharide (LPS)-induced RAW264.7 cells. Inflammation is classified with acute and chronic. Chronic inflammation is closely related to cause diseases, such as atherosclerosis, asthma, rheumatoid arthritis, inflammatory bowel disease and cancer [1]. Various attempts in medicinal herb have been demonstrated for prevention and treatment of inflammation. Corydalis bungeana Turcz showed anti-inflammatory effect by decreasing pro-inflammatory mediator including nitric oxide (NO), inducible nitric oxide synthesis (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) [2]. Viola yedoensis showed the anti-inflammatory effect on lipopolysaccharide (LPS)-induced RAW264.7 cells [3]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
