Abstract
A NEW SYSTEM HAS BEEN DEveloped for evaluating, in a single experiment, the identity, purity, and concentration of combinatorial library compounds made by parallel synthesis. In the system, high-performance liquid chromatography (HPLC) separates components of synthetic samples that are arrayed on microtiter plates. The eluent is first analyzed by a UV-visible photodiode array (PDA) detector and is then directed to three other analyzers—an evaporative light-scattering detector (ELSD), a chemiluminescent nitrogen detector (CLND), and a time-of-flight mass spectrometry (TOFMS) unit. The approach was developed by analytical chemists David A. Yurek and Ming-Shang Kuo and information scientist Derek L. Branch of the Discovery Technologies unit at Pharmacia Corp. [ J. Comb. Chem. , 4 , 138 (2002)]. It was devised to solve a common parallel synthesis problem: Target compounds are produced in a range of yields and purities, making it difficult to screen them for biological activity. Scientists generally address th...
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