Alleviation of ZnO nanoparticles-induced methanogenic inhibition by granular activated carbon

Abstract

ABSTRACT A great deal of attention has been paid to the toxicology of ZnO nanoparticles (NPs) to wastewater anaerobic digestion, but few studies have assessed how to reduce their toxic effects. In this study, different dosages of granular activated carbon (GAC) were added into ZnO NPs-bearing wastewater anaerobic digestion system. It was found that although the extracellular polymeric substances resistance, which had been impaired by ZnO NPs, could not be recovered by GAC, the suppressed methane yield was greatly enhanced by promoting the conversions of butyrate and acetate into methane. GAC of 10, 20 and 30 g/L enhanced the methane yield to 69%, 79% and 97% from 42% of the control inhibited by 100 mg/L ZnO NPs. This was mainly because the adsorption of GAC could weaken the effective contact between ZnO NPs and microbes, and also adsorb some released Zn2+ that has contributed primarily to ZnO NPs toxicology. The reduced toxicity of ZnO NPs was attributed to the enrichment of the tolerant hydrogenotrophic methanogens and the direct interspecies electron transfer-linking partners of Methanosarcina with Geobacter/Syntrophomonas. These syntrophic partners potentially used GAC as a conduit to transfer electrons for methane production.