Alleviation of microplastic toxicity in soybean by arbuscular mycorrhizal fungi: Regulating glyoxalase system and root nodule organic acid

Abstract

Microplastic accumulation in the soil-plant system can stress plants and affect products quality. Currently, studies on the effect of microplastics on plants are not consistent and underlying molecular mechanisms are yet unknown. Here for the first time, we performed a study to explore the molecular mechanism underlying the growth of soybean plants in soil contaminated with various types of microplastics (PS and HDPE) and arbuscular mycorrhizal fungi (AMF) (presence/absence). Our results revealed that a dose-dependent decline was observed in plant growth, chlorophyll content, and yield of soybean under MPs stress. The addition of MPs resulted in oxidative stress closely related to hydrogen peroxide generation (H2O2), methylglyoxal (MG) levels, lipid peroxidation (MDA), and lipoxygenase (LOX). In contrast, MPs addition enhanced mycorrhizal colonization and dependency relative to control while the rubisco and root activity declined. All the genes (GmHMA13 and GmHMA19) were downregulated in the presence of MPs except GmHMA18 in roots. AMF inoculation alleviated MPs-induced phytotoxic effects on colonization, rubisco activity, root activity and restored the growth of soybean. Under MPs exposure, AMF inoculation induced plant defense system via improved regulation of antioxidant enzymes, ascorbate, glutathione pool, and glyoxalase system. AMF upregulated the genes responsible for metals uptake in soybean under MPs stress. The antioxidant and glyoxalase systems coordinated regulation expressively inhibited the oxidative and carbonyl stress at both MPs types. Hence, AMF inoculation may be considered an effective approach for minimizing MPs toxicity and its adverse effects on growth of soybean grown on MPs-contaminated soils.