Alleviation of cadmium toxicity by improving antioxidant defense mechanism and nutrient uptake in wheat (Triticum aestivum L.) through foliar application of 24-epibrassinolide under elevated CO2.

Abstract

Heavy metals like cadmium (Cd) contamination occur in conjunction with the rising CO2 threatening food security and safety. Foliar application of 24-Epibrassinolide (EBR) was found to ameliorate Cd stress and improve nutrient availability in crops. However, its role under elevated CO2 is currently unknown. Accordingly, a pot experiment was conducted in open-top chambers (CO2 at 400 and 600 μmol mol−1) to determine the protective effect of EBR on wheat plants under different Cd concentrations (0, 2, and 4mgkg-1) in soil. The foliar application of EBR significantly improved growth, biomass, photosynthesis, proline, total phenol, and total soluble protein in Cd stress treatments under elevated CO2. Simultaneously, it significantly (p≤ 0.05) increased catalase (42.89%), superoxide dismutase (26.53%), peroxidase (28.10%), and ascorbate peroxidase (61.70%) while reduced malondialdehyde (35.53%), hydrogen peroxide (19.94%), and electrolyte leakage (23.55%) under elevated CO2 compared to ambient CO2 conditions. Furthermore, EBR and elevated CO2 interactively showed a maximum reduction in Cd concentrations and accumulation in the wheat roots (39.74,41.63%), shoots (46.83,44.87%), and grains (27.52,29.06%) respectively. Elevated CO2 and Cd stress interactively showed a significant reduction in nutrient content. Conversely, the EBR application recovered and significantly increased calcium, magnesium, iron, zinc, and copper content in wheat roots, shoots, and grains. Our findings inferred that EBR foliar application reduced Cd toxicity and improved plant growth and nutritional quality under elevated CO2.