Abstract
Background4-nitroquinoline 1-oxide (4-NQO) is a mutagen known to be responsible for causing cancer by generating oxidative stress in humans. Oroxylum indicum (L.) possesses various bioactive compounds with antioxidant properties. In this connection, the present study aims to analyze the alleviation of 4-NQO induced oxidative stress in albino Wistar rats using O. indicum (L.) leaf extract.MethodsO. indicum (L.) belonging to the family Bignoniaceae, has anticancer and anti-inflammatory properties. In this study, we observed severe oxidative stress in 4-NQO induced albino Wistar rats when compared to untreated control. Alleviation of this condition was seen after the oral administration of O. indicum (L.) leaf extract at 50, 100, and 200 mg/kg body weight.Results4-NQO (50 ppm) administration in drinking water resulted in the generation of reactive oxygen species (ROS) leading to cellular damage, lipid peroxidation and imbalance in antioxidant status. Administration of O. indicum (L.) leaf extract has alleviated the level of 4-NQO induced oxidative stress by increasing the antioxidant status and decreasing the elevation of liver markers in serum.ConclusionsResults clearly suggest that O. indicum (L.) leaf extract when administered orally in a dose dependent manner has the ability to overcome the oxidative stress induced by 4-NQO with hepatoprotective and lipid protective properties.
Highlights
4-nitroquinoline 1-oxide (4-NQO) (50 ppm) administration in drinking water resulted in the generation of reactive oxygen species (ROS) leading to cellular damage, lipid peroxidation and imbalance in antioxidant status
Evaluation of lipid peroxidation (LPO) in liver Figure 1 shows the effect of O. indicum (L.) leaf extract on thiobarbituric reactive acid substances (TBARS) level in liver of 4-NQO induced oxidative stress in rats
The results show a significant increase in TBARS level in the liver homogenate of group 2
Summary
Plant material Leaves of O. indicum (L.) were collected from Kottayam region, Kerala, India. The absorbance of pink color formation in the supernatant was measured at 535 nm. Antioxidant status in liver SOD activity, which is based on the inhibition of reduction of nitro blue tetrazolium (NBT) to blue formazan was measured according to Kakkar et al, (1984) [23] with modification. The absorbance of formazan in the supernatant was measured at 519 nm. The CAT activity in liver homogenate was carried out according to Sinha 1972 [24]. Determination of GPx level in liver was carried out according to Rotruck (1973) [25]. Reduced glutathione levels were assayed according to Ellman et al, (1961) [26]. The absorbance of GPx and GSH was measured at 650 nm. Vitamin E was assayed according to Baker and Frank (1988) [28]. Groups were considered statistically significant when p < 0.05 [29]
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