Abstract
AbstractTechnical advances in biotechnology lead to a rising number of genetically modified plants and derived food and feed. Therefore, the international interest in adequate and standardized analytical methods increases to assess health risks in a rapid and low cost manner.To address this issue a decision support system (DSS), which will be based on baseline data and thresholds of key parameters, is going to be established in the framework of a joint project (BioOK). Determination of the potential allergenicity or toxicity of novel proteins caused by the genetic modification compared to near isogenic variants (NIV) is a key issue in this context. For the development of DSS the model plant “potato” was used to compare values of relevant parameters of GM plants with those of NIV using different allergological and toxicological methods which are shown in the present poster. No significant differences in comparison of the results of GM plants and NIV were found so far.
Highlights
The experiments included various plant species (Zea mays, Solanum tuberosum, Vicia hirsuta, Salix and Populus spp.), which were grown in leaching vessels under controlled environmental conditions on different soils
Albatros): npt, ctb, vp[60], cphA - referring to the literature below), inoculation with mycorrhizal fungi (poplar (Populus nigra x maximowiczii)) and stages of plant development (Solanum tuberosum)
All rhizodeposits were leached at defined growth stages
Summary
Transgene effects on rhizodeposition: Evidence from molecular-chemical screening by Pyrolysis-Field Objective: Development of standard protocols to analyse rhizodeposits by Pyrolysis-Field Ionisation Mass Spectrometry (Py-FIMS) as an approach to detect possible influences of genetic modifications on soil microorganisms Sample introduction lyophilisate (rhizodeposit) volatilisation organic substances during pyrolysis
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