Abstract
BackgroundA neuroimmune crosstalk between dendritic cells (DCs) and airway nerves in the lung has recently been reported. However, the presence of DCs in airway sensory ganglia under normal and allergic conditions has not been explored so far. Therefore, this study aims to investigate the localisation, distribution and proliferation of DCs in airway sensory ganglia under allergic airway inflammation.MethodsUsing the house dust mite (HDM) model for allergic airway inflammation BALB/c mice were exposed to HDM extract intranasally (25 μg/50 μl) for 5 consecutive days a week over 7 weeks. With the help of the immunohistochemistry, vagal jugular-nodose ganglia complex (JNC) sections were analysed regarding their expression of DC-markers (MHC II, CD11c, CD103), the neuronal marker PGP 9.5 and the neuropeptide calcitonin gene-related peptide (CGRP) and glutamine synthetase (GS) as a marker for satellite glia cells (SGCs). To address the original source of DCs in sensory ganglia, a proliferation experiment was also carried in this study.ResultsImmune cells with characteristic DC-phenotype were found to be closely located to SGCs and vagal sensory neurons under physiological conditions. The percentage of DCs in relation to neurons was significantly increased by allergic airway inflammation in comparison to the controls (HDM 51.38 ± 2.38% vs. control 28.16 ± 2.86%, p < 0.001). The present study also demonstrated that DCs were shown to proliferate in jugular-nodose ganglia, however, the proliferation rate of DCs is not significantly changed in the two treated animal groups (proliferating DCs/ total DCs: HDM 0.89 ± 0.38%, vs. control 1.19 ± 0.54%, p = 0.68). Also, increased number of CGRP-positive neurons was found in JNC after allergic sensitisation and challenge (HDM 31.16 ± 5.41% vs. control 7.16 ± 1.53%, p < 0.001).ConclusionThe present findings suggest that DCs may migrate from outside into the ganglia to interact with sensory neurons enhancing or protecting the allergic airway inflammation. The increase of DCs as well as CGRP-positive neurons in airway ganglia by allergic airway inflammation indicate that intraganglionic DCs and neurons expressing CGRP may contribute to the pathogenesis of bronchial asthma. To understand this neuroimmune interaction in allergic airway inflammation further functional experiments should be carried out in future studies.
Highlights
Allergic bronchial asthma is a chronic inflammatory respiratory disease characterised by airway obstruction, bronchial hyperreactivity and airway inflammation with the recruitment of a variety of immune cells including dendritic cells (DCs) [1,2,3]
Under physiological and allergic conditions, DCs were observed to be widely distributed over the whole jugular-nodose ganglia complex (JNC) and were located between the neurons and nerve fibres which were immunoreactive for PGP 9.5 (Figure 3)
The present study revealed for the first time the existence of immune cells with DC phenotype within JNC
Summary
Allergic bronchial asthma is a chronic inflammatory respiratory disease characterised by airway obstruction, bronchial hyperreactivity and airway inflammation with the recruitment of a variety of immune cells including dendritic cells (DCs) [1,2,3]. In the allergic sensitisation phase, DCs play a key role as professional antigen presenting cells in the allergic airway inflammation [3,4,6] They capture the antigen, process and subsequently present it on the MHC class II molecules (MHC II) to naïve T lymphocytes in local lymph nodes leading to cascades of the Th2-immune allergic inflammatory processes [4,7,8]. CGRP released from airway nerve fibres has the capacity to act as chemoattractant factor for different immune cells such as CD4+ T-lymphocytes, CD8+ T-lymphocytes, eosinophils and DCs and to induce the proliferation of airway epithelial cells [9,15,16,17,18,19]. This study aims to investigate the localisation, distribution and proliferation of DCs in airway sensory ganglia under allergic airway inflammation
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