Allelopathy of the raphidophyte Heterosigma akashiwo against the dinoflagellate Akashiwo sanguinea is mediated via allelochemicals and cell contact

Abstract

Growth interactions between the raphidophyte Heterosigma akashiwo and the dinoflagellate Akashiwo sanguinea were examined by using bi-algal culture experiments under axenic conditions. There was a lethal effect of H. akashiwo (initial cell density: 1 × 10 2 or 1 × 10 4 cells ml �1 ) on A. sanguinea at an initial cell density of 1 × 10 2 cells ml �1 . Growth of both species was suppressed concurrently when initial cell densities of H. akashiwo and A. sanguinea were 1×1 0 2 and 2 × 10 3 cells ml �1 , respectively. A mathematical model was used to simulate growth and interactions in bi-algal cultures. The model predicted that H. akashiwo would outcompete A. san- guinea over time. A cell-free enriched filtrate prepared from dense culture of H. akashiwo reduced both maximum growth rate and maximum yield of A. sanguinea. A filtrate of A. san- guinea prepared in the same manner only reduced maximum yield of H. akashiwo but did not affect its maximum growth rate. Growth of A. sanguinea in bi-algal cultures under contact condi- tions was significantly lower than that in mono-algal culture and in bi-algal cultures under non- contact conditions. Morphologically abnormal A. sanguinea cells occurred at high frequency only when A. sanguinea was cultured together with H. akashiwo under direct cell contact condition. Moreover, growth inhibition and formation of morphologically abnormal cells of A. sanguinea were induced in a concentration-dependent manner by allelochemicals (including allelochemical polysaccharideprotein complexes) produced by H. akashiwo. These results suggest that growth inhibition effects, via allelochemicals and direct cell contact by Heterosigma akashiwo, influence bloom formation of Akashiwo sanguinea in the field.