Abstract
Since Populus has veritable value as timber, plywood, pulp, and paper, genomic research should create the sound basis for further breeding toward desirable wood quality attributes. In this study, we addressed the need for a research methodology that initially identifies and then characterize candidate genes encoding enzymes with wood property phenotypic traits, toward the aim of developing a genomics-based breeding technology. On 23 different poplar species/hybrid samples, we successfully amplified 55 primers designed on Populus trichocarpa L. Considering the number of polymorphic sites, out of 73,206 bp, 51 SNPs and 31 indel events were found. Non-synonymous single base mutations could be detected in number of 30, 21 out of 164 sequences were the number of minimum recombination events and 41 significant pairwise comparisons between loci could be detected. Our results provide a roadmap for a future association genetic study between nucleotide diversity and precise evaluation of phenotype.
Highlights
Since Populus has veritable value as timber, plywood, pulp, and paper, genomic research should create the sound basis for further breeding toward desirable wood quality attributes
To localize the insertion event with the aim to determine which sequence and where has been disrupted, we created a second ambiguity sequence, this ambiguity sequence being aligned to an assembled Populus genome sequence
The genomic sequence that best aligned to the ambiguity sequence was assumed to be the second sequence of the same sample
Summary
Since Populus has veritable value as timber, plywood, pulp, and paper, genomic research should create the sound basis for further breeding toward desirable wood quality attributes. Materials and methods: In this study, we addressed the need for a research methodology that initially identifies and characterize candidate genes encoding enzymes with wood property phenotypic traits, toward the aim of developing a genomics-based breeding technology. Hybrid poplars are important components of current energy portfolios for their significant amount of biomass, but they provide a wide range of wood products, including industrial roundwood and poles, pulp and paper, reconstituted boards, plywood, veener, sawn timber, packing crates, pallets, and furniture. In the near future, densified poplars may be used for flooring or as furniture elements, complementing or replacing high-density hardwoods on the wood market (Rademacher et al, 2017)
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