ALLELIC STATUS OF THE Ppd-1 AND VRN-1 GENETIC SYSTEMS IN WINTER WHEAT VARIETIES OF THE INSTITUTE OF IRRIGATED AGRICULTURE OF NAAS UKRAINE

Abstract

The aim of the current investigation was to determine the alleles of the genes of Ppd-1 and Vrn-1 systems in 13 bread winter wheat varieties created in the Institute of Irrigated Agriculture of the National Academy of Sciences of Ukraine (IIA) and to compare the data of the molecular genetic analysis with the data of field research. To accomplish this goal, the following tasks were set: 1) to determine the allelic state of the genes of the systems Ppd-1 and Vrn-1 in the genotypes of bread wheat varieties created in the IIA; 2) to collect the data of observations at stages of development (heading and flowering) of wheat varieties in the conditions of the Southern steppe of Ukraine and (3) to carry out a statistical analysis of the dependence of the periods of heading and flowering on the allelic composition of genes Ppd-1 and Vrn-1 in the genotypes of wheat varieties. Material. Varieties of winter bread wheat created in IIA. Methods. DNA extraction, allele-specific and nested PCR, electrophoresis of amplification products, one-way dispersion analysis (ANOVA). Results . By the Ppd-1 system in all IIA varieties dominant Ppd-D1a allele was detected, recessive alleles b were identified in Ppd-A1 and Ppd-B1 loci. The combination of mutations in the nucleotide sequence of the Ppd-D1 gene indicates that the studied varieties belong to the haplotype VII. By the Vrn-1 system, the varieties are carriers of a recessive genotype. No increase in the number of Ppd-B1 gene copies was detected in the genotype of the variety. The varieties investigated are early and do not have any significant differences in terms of heading and flowering, which corresponds to the results of the molecular-genetic analysis of Ppd-1 and Vrn-1 genes that control the duration of the vegetative period of wheat plants.