Allelic sharing among madurese as a tool of madurese identification using 11 short tandem repeats and amelogenin gene: An observational analytical study

Abstract

Aim: To analyze the allelic sharing and a set of preferable short tandem repeats (STRs) loci among the Madurese full siblings. Materials and Methods: This was an observational analytical study. The research subjects were determined from 20 Madurese families that originated from Madura Island, Indonesia consisting of father, mother, and two biological children. The blood samples were collected, and kinship relationship was examined by using the PCR-STR technique by 11 loci of genotyped STR alleles and a sex-typing amelogenin locus. Calculations of combined sibship indices (CSIs) for each pair were done to determine the likelihood ratios. Results: Based on 220 observations (20 pairs of full sibling x 11 loci) of the STR allele genotype, 4 STR loci were found: D8S1179, F13, CSF1PO, and FES. A high-shared allelic frequency was found in two alleles that were shared among 11 STR loci by 57 times (25.9%), one allele shared among eleven STR loci by 130 times (59.1%), and zero alleles by 33 times (15.0%). Conclusion: This study indicated the power of one allele in the sibship among a Madurese establishment through the 11 STR loci.