Abstract
Chalcone synthase (CHS) is an essential enzyme in the phenylpropanoid pathway that catalyzes the first step in flavonoid biosynthesis in plants under diverse environmental stress. We have used CHS as a candidate gene in mulberry and developed Single Nucleotide Polymorphism (SNP) based co-dominant Cleaved Amplified Polymorphic Sequence (CAPS) marker associated with the CHS locus. The segregation pattern of the marker was studied in an F1 population derived from a hybridization program between two mulberry genotypes showing polymorphism for the CHS locus. Differential CHS activity of the recombinants has been correlated with the segregation pattern of the marker. Homology modelling and docking studies are performed for both the identified CHS alleles and correlated with respective CHS activity. Phenotyping of Powdery Mildew infected F1 population indicated a probable association with the CAPS marker.
Highlights
Mulberry (Morus spp., family Moraceae) foliage is the only forage for the silkworms (Bombyx mori L.)
The sequences were used for conserved domain search [22,23,24,25], and it showed that the Chalcone synthase (CHS) gene showed highly conserved amino acids in the active site as well as in the substrate binding site in the three varieties
We have demonstrated the presence of two allelic form of CHS gene in an F1 recombinant population of a mulberry hybridization program
Summary
Mulberry (Morus spp., family Moraceae) foliage is the only forage for the silkworms (Bombyx mori L.). It is the most important plant from sericulture point of view. In recent years the cultivation of mulberry is declining very fast due to preferences for growing of cereals and other high-value crops in the shrinking arable land. Stressrelated yield loss of mulberry ranges from 50% to 60% [2,3]. The solution to this problem lies in the development of superior mulberry genotypes having tolerance to biotic and abiotic stresses vis-à-vis higher yield. The dioecious nature coupled with long juvenile period and high heterozygosity acts as the significant impediment to developing inbred lines in mulberry [4]
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