Allele Frequency of HLADQB1 Locus in Macedonian Population

Abstract

Aim: The aim of this paper was to genotype HLA DQB1 locus in healthy unrelated Macedonian population. Material and Methods: Reverse Line Strip typing for HLA DQB1 locus was performed on a population of 217 samples from healthy individuals. The results were obtained as alleles, and as NMDP Codes. Alleles were genotyped and expressed as high resolution with more than four digits with slash between ambiguous alleles. NMDP Codes were expressed as four digits for unambiguous results, and with combination of numbers and characters for the certain ambiguous combination. We did not found any genotypic ambiguities in HLA-DQB1. Results: We have found 33 different alleles of HLA DQB1 in Macedonian population, 12 of which were unambiguous with the frequency of 40.55%, and 21 were ambmiguous with the frequency of 58.53%. The biggest frequency (33.64%) was found for HLA DQB1*05 unambiguous groups, and for HLA DQB1*03 ambiguous groups (36.40%). The biggest allele frequency in Macedonian population for HLA DQB1 was found for HLA DQB1*03NX (0301/01/01/02/09/13) with 27.88%, followed with HLA DQB1*0502 with 13.82%, and HLA DQB1*02MN (0201/02/03) with 10.37%. Conclusion: Allele frequency of HLA DQB1 in Macedonian population is similar with that found in other European populations and can be used for anthropology and disease association studies.