Abstract
All-optical interrogation of population neuron activity is a promising approach to deciphering the neural circuit mechanisms supporting brain functions. However, this interrogation is currently limited to local brain areas. Here, we incorporate patterned photo-stimulation into light-sheet microscopy, allowing simultaneous targeted optogenetic manipulation and brain-wide monitoring of the neuronal activities of head-restrained behaving larval zebrafish. Using this system, we photo-stimulate arbitrarily selected neurons (regions as small as ~10-20 neurons in 3D) in zebrafish larvae with pan-neuronal expression of a spectrally separated calcium indicator, GCaMP6f, and an activity actuator, ChrimsonR, and observe downstream neural circuit activation and behavior generation. This approach allows us to dissect the causal role of neural circuits in brain functions and behavior generation.
Highlights
Large-scale activation of neural circuits across the whole brain is often required to generate animal behaviors [1,2,3,4]
We selected single neurons in the zebrafish brain from the fluorescent images and patterned the photo-stimulation based on the digital micromirror device (DMD) pixel registration to the sCMOS (Fig. 3 and Fig. 7(a))
This is critical for elucidating brain-wide neural circuit mechanisms supporting animal behaviors, because behaviorassociated neuronal ensembles are usually distributed across many brain areas [1,2,3,4,5,6,7]
Summary
Large-scale activation of neural circuits across the whole brain is often required to generate animal behaviors [1,2,3,4]. The larval zebrafish is an ideal animal model for such studies, because its small and transparent brain is well suited to optical monitoring and manipulation of neuronal activities across the whole brain at single-cell resolution [6,8]. This good candidacy is especially true considering the relatively recent technological advances concerning probes for optical readout and manipulation of neuronal activity [9,10,11,12]. Only hundreds of neurons in local brain areas were examined simultaneously, because of the limitation of the point imaging assay [13,14,15], which prevented examination of brain-wide neural circuits
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
