ALKBH5 Is Lowly Expressed in Esophageal Squamous Cell Carcinoma and Inhibits the Malignant Proliferation and Invasion of Tumor Cells.

Jinqiu Li,Jing Wang,Shanglin Dong,Yunbo Zhang,Xiao Li,Hongqiang Liu

doi:10.1155/2021/1001446

Jinqiu Li, Jing Wang + Show 4 more

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https://doi.org/10.1155/2021/1001446

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Abstract

Background Modification of N6-methyladenosine (m6A) and RNA m6A regulatory factors is required in cancer advancement. The contribution of m6A and its alteration in esophageal squamous cell carcinoma (ESCC) is still unclear. Results ALKBH5 was lowly expressed in ESCC tissues, which the total m6A level was increased in ESCC tissue than the presentation in normal healthy tissue. The pcDNA3.1-ALKBH5 recombinant plasmid was transfected into KYSE-150 and Eca-109 cells. The overexpression of ALKBH5 is responsible for a significant reduction of the total m6A levels in Eca-109 and KYSE150 cells, inhibiting the proliferation capability, migration, and cell invasion. Conclusions ALKBH5 as a demethylase was lowly expressed in cancer progression of ESCC and acts as a crucial component in ESCC progression.

Highlights

Modification of N6-methyladenosine (m6A) and RNA m6A regulatory factors is required in cancer advancement
The presentation of gene expression of ALKBH5 was measured in 23 esophageal squamous cell carcinoma (ESCC) tissue samples using qRT-PCR and Immunohistochemistry Staining Analysis (IHC)
The results of IHC showed that ALKBH5 had abundant protein expression in normal esophageal tissues (Figure 1(b))

Summary

Background

Esophageal cancer (EC) ranks seventh in the global cancer incidence rate and sixth cause of cancer mortalities, globally [1]. In China, EC accounts for the majority of cancer mortalities, among which ESCC is the leading subtype (80%) [2]. China’s ESCC cases account for half of the burden of the global ESCC patients and more than 90% of Asia’s [3]. The survival rate in ESCC cases is still very low [5]. N6-Methyladenosine (m6A) is referred to as a modification of RNA in mammals [6]. “Readers” (YTHDF1/2/3, YTHDC1/2, HNRNPA2/B1, HNRNPC, HNRNPG, eIF3, IGF2BP1/2/3, and Prrc2a) can selectively recognize m6A modified RNA and perform specific biological functions. Guo et al identify the prognostic characteristics of EC patients including ALKBH5 and HNRNPA2B1 [10]. We will clarify the function of ALKBH5 in the malignant growth and invasion of ESCC cells in vitro

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