Abstract
Summary Growth, alkaloid formation and enzyme activities of some shikimic acid pathway enzymes were examined in cell suspension cultures of Ruta graveolens. Aromatic amino acids inhibit slightly the activity of 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase indicating a weak allosteric regulation. Feedback inhibition of chorismate mutase by phenylalanine and tyrosine as well of anthranilate synthase by tryptophan was observed. Tryptophan at 1mM stimulated the activity of chorismate mutase and reversed the Phe and/or Tyr inhibition of CM. Tissue cultures of Ruta graveolens were grown in liquid media containing L-tryptophan. The levels of DAHP-synthase and tryptophan synthase were elevated in these cells. A slight repression of anthranilate synthase was observed in 4 days old cells which were grown in media containing upto 1.5 mM tryptophan. A remarkable decrease of AA-Sase activity was found in 10 days old cells cultivated in the presence of tryptophan. A low alkaloid producing R. graveolens cell line was cultivated in the presence of exogenously added rutacridone and the influence of this particular acridone alkaloid on growth and shikimate pathway enzymes was investigated.
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