Alkaloid neurotoxins-dependent sodium transport in insect synaptic nerve-ending particles

Abstract

1. 1. Sodium uptake associated with the activation of voltage-sensitive sodium channels by alkaloid activators, batrachotoxin, veratridine, and aconitine in presynaptic nerve terminals isolated from the central nervous system of cockroach ( Periplaneta americana) was investigated. 2. 2. Batrachotoxin ( K 0.5, 0.2 μM) was full agonist as for most effective activator of Na + uptake; veratridine ( K 0.5, 2.5 μM) and aconitine ( K 0.5, 7.6 μM) produced a maximal stimulation of 22Na + uptake that were 71% and 43% respectively of that produced by batrachotoxin. 3. 3. Veratridine-dependent 22Na + uptake was completely inhibited by tetrodotoxin ( I 0.5, 11 nM), a specific inhibitor of the nerve membrane sodium channels. 4. 4. The present study describes appropriate conditions for measuring neurotoxins — stimulated sodium transport in insect central nervous system synaptosomes. The data show that voltage-sensitive sodium channels as defined by specific activation by the alkaloid neurotoxins are qualitatively distinct in insect synaptosomes than those previously described for vertebrate brain synaptosomes, cultured neuronal cell, nerve membrane vesicles and neuroblastoma cells.