Abstract
O-Acetylvallesamine is the main alkaloid found in both medium and cells of the Tabernaemontana divaricata cell suspension culture investigated. [ 15N] O-Acetylvallesamine was added to the culture medium. Rapid exchange between biomass and culture medium was shown by the rapid distribution of labelled compound over the two compartments. The occurrence of biosynthesis throughout most of the investigated period was confirmed by the observed dilution of the labelled compound. Transformation, calculated from the decrease in the total amount of I 15N] O-acetylvallesamine, was also observed and occurred mainly during the stationary phase of the batch cell culture growth cycle.
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